CD8 antibody (FITC)

Catalog No. ABIN181943

The Mouse Monoclonal anti-CD8 antibody has been validated for FACS. It is suitable to detect CD8 in samples from Sheep.

Quick Overview for CD8 antibody (FITC) (ABIN181943)

Target: CD8

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 38-65

Product Details anti-CD8 Antibody (FITC)

Specificity: This antibody recognises the CD8 cell surface antigen, which is expressed by the cytotoxic/suppressor subset of T lymphocytes.

Purification: Affinity chromatography on Protein G

Immunogen: Ovine efferent lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 281060

NCBI Accession: NP_776440

UniProt: P31783